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[1]张海宽,贾 宁,冷艾泠.高分辨CT、胸部平片及病理学诊断肺磨玻璃结节临床分析[J].中华肺部疾病杂志,2020,(03):360-364.[doi:10.3877/cma.j.issn.1674-6902.2020.03.014]
　Li Shubin,Yu Hong,Zhang Gengyue..Differential expression of mRNA and lncRNA in erlotinib-resistant non-small cell lung cancer cells[J].,2020,(03):360-364.[doi:10.3877/cma.j.issn.1674-6902.2020.03.014]
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高分辨CT、胸部平片及病理学诊断肺磨玻璃结节临床分析(PDF)

《中华肺部疾病杂志》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年03期
页码:
360-364
栏目:
论著
出版日期:
2020-06-20

文章信息/Info

Title:
Differential expression of mRNA and lncRNA in erlotinib-resistant non-small cell lung cancer cells
作者:
张海宽; 贾 宁; 冷艾泠
401120 重庆,重庆医科大学附属第三医院(捷尔医院)健康管理中心
Author(s):
Li Shubin1;  Yu Hong2;  Zhang Gengyue2.
1Department of Internal Medicine 1, South District, Guang'anmen Hospital, Chinese Academy of Traditional Chinese Medicine, Beijing 102600, China; 2Department of Cell Biology, Institute of Cancer Prevention and Control, Jilin Provincial Cancer Hospital, Changchun 130012, China
关键词:
肺磨玻璃结节;  高分辨CT;  胸部平片;  病理学;  鉴别诊断
Keywords:
Non-small cell lung cancer;  Erlotinib drug resistance;  Differential expression;  IncRNA analysis
分类号:
R563
DOI:
10.3877/cma.j.issn.1674-6902.2020.03.014
摘要:
目的 筛选并分析厄洛替尼耐药的非小细胞肺癌细胞的差异表达mRNAs和lncRNAs。方法 在Gene Expression Omnibus(GEO)数据库中挑选数据集GSE80344,其数据来自厄洛替尼耐药的HCC827细胞和敏感细胞。通过GEO2R和探针匹配分析差异表达mRNAs和lncRNAs,筛选标准为|Fold change|>1.5,P value<0.01。通过Panther(http://www.pantherdb.org)、KEGG(http://www.kegg.jp/)、STRING(http://string-db.org)等数据库分析筛选得到的差异表达mRNAs和lncRNAs的可能的作用机制。通过TCGA(https://cancergenome.nih.gov/)数据库对筛选得到的关键mRNAs和lncRNAs进行生存分析验证。结果 厄洛替尼耐药的HCC827细胞和敏感细胞相比,差异表达mRNAs共644个,差异表达lncRNAs共367个,其中128 mRNAs表达上调,516 mRNAs表达下调; 137 lncRNAs表达上调,230 lncRNAs表达下调。这些mRNAs和lncRNAs主要涉及在PI3K-Akt、Ras、MAPK、ECM受体相互作用等与癌症密切相关的信号通路中发挥作用。基于数据库的分析验证结果与筛选结果一致。结论 筛选分析得到的关健mRNAs和lncRNAs可为深入研究非小细胞肺癌EGFR-TKIs耐药机制的前期实验依据。
Abstract:
Objective To identify the differential expression of mRNA and lncRNA in the erlotinib-resistant non-small cell lung cancer cells. Methods GSE80344 microarray datasets were selected from the Gene Expression Omnibus(GEO)database including the data from erlotinib-resistant HCC827 cells and sensitive cells. The aberrantly-expressed mRNAs and lncRNAs were analyzed by GEO2R and probe ID matching. The screening criteria were |Fold change|>1.5 and P value<0.01. The possible mechanisms of action of dysregulatory mRNAs and lncRNAs were investigated by Panther(http://www.pantherdb.org), KEGG(http://www.kegg.jp/), STRING(http://string-db.org), and other databases. The candidate key mRNAs and lncRNAs obtained by screening were verified by survival analysis using the data from the TCGA database(https://cancergenome.nih.gov/). Results Compared with the sensitive cells, there were 644 differentially-expressed mRNAs and 367 differentially-expressed lncRNAs in the erlotinib-resistant HCC827 cells, respectively, of which 128 mRNAs were up-regulated and 516 mRNAs were down-regulated. And 137 lncRNAs were up-regulated and 230 lncRNAs were down-regulated. These mRNAs and lncRNAs were mainly involved in the signaling pathways closely related to cancers, such as PI3K-Akt, Ras, MAPK, ECM receptor interactions, and so on. Database-based verification confirmed the screening results. Conclusion The screened out mRNAs and lncRNAs can provide theoretical and preliminary experimental bases for the further study on the mechanisms of EGFR-TKIs resistance of non-small cell lung cancer.

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备注/Memo

备注/Memo:
通信作者: 贾 宁, Email: 369406800@qq.com
更新日期/Last Update: 2020-06-20