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[1]许 娟,张党锋.尼达尼布对肺纤维化小鼠肺功能及内质网应激反应的影响[J].中华肺部疾病杂志,2023,(05):673-675.[doi:10.3877/cma.j.issn.1674-6902.2023.05.016]
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尼达尼布对肺纤维化小鼠肺功能及内质网应激反应的影响(PDF)

《中华肺部疾病杂志》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年05期
页码:
673-675
栏目:
临床研究
出版日期:
2023-10-20

文章信息/Info

Title:
-
作者:
许 娟1; 张党锋2
710077 西安,西安外事学院1
710061 西安,西安交通大学第一附属医院骨科2
Author(s):
-
关键词:
肺纤维化;  尼达尼布;  肺功能;  内质网应激反应
Keywords:
-
分类号:
R332
DOI:
10.3877/cma.j.issn.1674-6902.2023.05.016
摘要:
目的 观察尼达尼布对肺纤维化小鼠肺功能及内质网应激反应的影响。方法 从36只小鼠中随机抽取12只为对照组,其余24只构建肺纤维化模型,随机分为模型组12只、尼达尼布组12只。术后14 d,尼达尼布组灌胃尼达尼布100 mg·kg-1,对照组、模型组灌胃等体积生理盐水溶液。每天1次,连续14 d。末次干预后4 h检测小鼠肺功能; 免疫印迹法检测肺组织葡萄糖调节蛋白78(GRP78)、激活转录因子6(ATF6)蛋白表达量。结果 对照组、模型组、尼达尼布组用力肺活量(FVC)分别为(10.24±1.10)ml、(6.35±0.72)ml、(8.41±0.95)ml,第0.3秒用力呼气量(FEV0.3)分别为(7.68±0.89)ml、(3.37±0.56)ml、(5.20±0.74)ml; 3组的GRP78蛋白表达量分别为(0.12±0.02)、(0.39±0.04)、(0.22±0.03),ATF6蛋白表达量分别为(0.23±0.04)、(0.53±0.06)、(0.40±0.05)。模型组与对照组比较,尼达尼布组与模型组比较,差异有统计学意义(P<0.05)。结论 尼达尼布可改善肺纤维化小鼠肺功能,降低肺部炎性程度及纤维化程度,下调内质网应激相关蛋白GRP78、ATF6、p-PERK表达。
Abstract:
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参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 陕西省自然科学基础研究计划项目(2019JM-562)
通信作者: 张党锋, Email: 13772491392@163.com
更新日期/Last Update: 2023-10-20