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[1]林 英,何 洪,杨 琦,等.联合宏基因组二代测序诊断普雷沃氏菌致肺脓肿1例并文献复习[J].中华肺部疾病杂志,2024,(04):625-629.[doi:10.3877/cma.j.issn.1674-6902.2024.04.024]
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联合宏基因组二代测序诊断普雷沃氏菌致肺脓肿1例并文献复习(PDF)

《中华肺部疾病杂志》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年04期
页码:
625-629
栏目:
出版日期:
2024-08-25

文章信息/Info

Title:
-
作者:
林 英1; 何 洪1; 杨 琦1; 姚兴伟1; 马婧涵1; 杨玉婷1; 刘月红1; 贾 悦1; 李长安2
100700 北京,北京中医药大学东直门医院检验科1、呼吸科2
Author(s):
-
关键词:
肺脓肿;  普雷沃氏菌;  宏基因组二代测序
Keywords:
-
分类号:
R563
DOI:
10.3877/cma.j.issn.1674-6902.2024.04.024
摘要:
目的 分析普雷沃菌(Prevotella)致肺脓肿鉴定诊断和治疗思路。方法 选择我院收治的1例肺脓肿患者,经厌氧培养,采用全自动微生物质谱检测系统 Autof ms1000 、16S 核糖体 DNA(ribosomal DNA, rDNA)基因序列分析、胸腔脓液、宏基因二代测序(metagenomic nextgeneration sequencing, mNGS)明确病原菌,进行文献复习。结果 采用全自动微生物质谱检测系统 Autof ms1000鉴定菌株为普雷沃菌,16S rDNA基因序列分析显示解肝素普雷沃菌(NR044633.1)98.517%, mNGS 明确病原菌为普雷沃氏菌属26%,梭杆菌属13%,棒杆菌属7%。予哌拉西林钠舒巴坦钠3 g 联合奥硝唑0.5 g/d二次静点抗感染,症状明显好转,无发热,无咳嗽咳痰,复查胸部CT右下肺高密度影基本吸收。结论 普雷沃菌是一种条件致病厌氧菌,多发生在机会性感染,易误诊漏诊,建议尽早进行mNGS明确病原体。经典微生物培养联合宏基因二代测序技术可提高肺部感染性疾病的病原微生物检出率,为精准抗菌治疗提供临床依据。
Abstract:
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参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金资助项目(81973784)
通信作者: 李长安, Email: lichangan1989@163.com
更新日期/Last Update: 2024-08-25