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[1]黄波翠,蔡思铭,古裕鸟,等.哮喘患儿IL-10基因多态性与肺功能及外周血Treg细胞的相关性[J].中华肺部疾病杂志,2024,(06):1003-1007.[doi:10.3877/cma.j.issn.1674-6902.2024.06.029
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哮喘患儿IL-10基因多态性与肺功能及外周血Treg细胞的相关性(PDF)

《中华肺部疾病杂志》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年06期
页码:
1003-1007
栏目:
临床研究
出版日期:
2024-12-25

文章信息/Info

Title:
-
作者:
黄波翠; 蔡思铭; 古裕鸟; 庄秀娟; 钟娇霞; 吴小文; 霍开明
570100 海口,海南医科大学第二附属医院儿科二区
Author(s):
-
关键词:
支气管哮喘;  白细胞介素-10;  单核苷酸多态性;  肺功能;  调节性T细胞;  免疫应答
Keywords:
-
分类号:
R562
DOI:
10.3877/cma.j.issn.1674-6902.2024.06.029
摘要:
目的 分析哮喘患儿白介素-10(interleukin-10, IL-10)基因单核苷酸多态性(single nucleotide polymorphism, SNP)与肺功能及外周血调节性T细胞(regulatory cell, Treg)水平的相关性。 方法 选取2021年2月至2024年2月我院收治的57例哮喘患儿为对象,病情严重程度中度33例为观察组和轻度24例对照组,采用实时聚合酶链反应(polymerase chain reaction, PCR)对两组的4种IL-10 基因SNP位点进行基因分型,检测外周血IL-10及单核细胞中Tregs细胞水平、肺功能情况,采用Pearson相关性分析IL-10基因多态性与肺功能及外周血Treg细胞水平的相关性。 结果 观察组第1秒用力呼气容积(forced expiratory volume in one second, FEV1)(27.45±3.56)% 、用力肺活量(forced vital capacity, FVC)(39.40±5.81)%及FEV1/FVC 比值(66.29±5.42)%低于对照组FEV1(31.48±2.93)%、FVC[(43.42±6.02)%及FEV1/FVC比值(75.15±6.13)%(P<0.05); 观察组IL-10水平(94.83±11.46)pg/ml、Tregs细胞(1.85±0.54)%低于对照组IL-10水平(105.43±15.20)pg/ml、Tregs细胞(2.43±0.61)%(P<0.05)。观察组IL-10基因rs3024491位点的TT基因型占比75.00%,两组IL-10基因rs3024496、rs3024491位点基因型的分布频率差异具有统计学意义(P<0.05); 观察组不同IL-10基因 rs3024491位点基因型分型IL-10水平、外周血Treg细胞水平及FEV1/FVC比值差异具有统计学意义(P<0.05); Pearson相关性分析显示,IL-10基因 rs3024491位点的TT基因型与IL-10及FEV1/FVC比值呈正相关(r=0.508,P=0.000; r=0.379,P=0.000),与外周血Treg细胞水平呈负相关(r=-0.446,P=0.000)。 结论 中度哮喘患儿中IL-10基因 rs3024491位点多态性频率高,TT基因型与肺功能、IL-10降低和Tregs细胞增加相关,影响患儿哮喘严重程度。
Abstract:
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备注/Memo

备注/Memo:
通信作者: 霍开明, Email: 18789909188@139.com
更新日期/Last Update: 2024-12-25