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《中华肺部疾病杂志》[ISSN:1006-6977/CN:61-1281/TN]

卷:

期数:

2025年02期

页码:

279-283

栏目:

论著

出版日期:

2025-04-25

文章信息/Info

Title:

Inhibitory effect of plasma-activated physiological saline on Mycobacterium tuberculosis in vitro

作者:

王 卓; 屈少毅; 邹远妩; 仵倩红; 李 静; 王 彪; 魏子涵; 王晓琳

710105 西安,陕西省结核病防治院

Author(s):

Wang Zhuo;  Qu Shaoyi;  Zou Yuanwu;  Wu Qianhong;  Li Jing;  Wang Biao;  Wei Zihan;  Wang Xiaolin.

Shaanxi Provincial Tuberculosis Prevention and Control Hospital, Xian 710105, China

关键词:

结核分枝杆菌;  离子活化生理盐水;  抑菌作用

Keywords:

Mycobacterium tuberculosis;  Plasma-activated physiological saline;  Bacteriostatic action

分类号:

R562

DOI:

10.3877/cma.j.issn.1674-6902.2025.02.014

摘要:

目的 分析等离子活化生理盐水(plasma-activated physiological saline, PAS)对结核分枝杆菌的体外抑菌作用及临床意义。方法 选择1株结核分枝杆菌标准菌株和5株临床分离的结核分枝杆菌,编号6180、6943、7158、 6545、7154开展生长实验,采用生理盐水为NS组、等离子体活化生理盐水为PAS组和盐酸为HCl组处理结核分枝杆菌,观察培养31 d后每组结核分枝杆菌落形态,统计培养第14天、第20天、第26天、第31天后细菌数量。绘制生长曲线,采用Kruskal-Wallis检验比较组间菌落生长差异。结果 观察菌落形态显示,结核分枝杆菌培养14 d,除6180菌株,其余5株菌NS组有菌落生长,菌落数50～100个; 结核分枝杆菌培养20 d,HCl组6株菌落生长; 结核分枝杆菌培养26 d,除 6545菌株的PAS组,其余5株菌有生长; 结核分枝杆菌培养31 d, 6株菌NS组和HCl组菌落大小、形状及菌落分布基本一致,PAS组中菌落分布较少; 生理盐水、PAS、HCl处理条件下6株结核分枝杆菌生长曲线结果显示,PAS组生长趋势低于NS组和HCl组; 生理盐水、PAS、HCl处理条件下结核分枝杆菌菌落生长差异比较,NS组结核分枝杆菌的菌落计数高于PAS组(P<0.05),NS组和HCl组结核分枝杆菌的生长情况差异无统计学意义(P>0.05)。结论 等离子活化生理盐水在体外实验中对结核分枝杆菌有抑制作用,对抗结核治疗展现出较大潜力,联合治疗发挥协同作用,以提高临床疗效。

Abstract:

Objective To analyze the in vitro bacteriostatic effect and clinical significance of plasma activated saline(PAS)on Mycobacterium tuberculosis. Methods One standard strain of Mycobacterium tuberculosis and five clinically isolated mycobacterium tuberculosis(No. 6180, 6943, 7158, 6545, 7154)were selected for growth experiments. Mycobacterium tuberculosis was treated with normal saline(NS group), plasma activated saline(PAS group)and hydrochloric acid(HCl group). The colony morphology of Mycobacterium tuberculosis in each group was observed after 31 days of culture, and the number of bacteria was counted after 14, 20, 26 and 31 days of culture. The growth curve was drawn, and Kruskal-Wallis test was used to compare the differences in colony growth between groups. Results Colony morphology of Mycobacterium tuberculosis was observed. After 14 days of culture, except 6180 strains, the other 5 strains had colony growth in NS group, with 50～100 colonies. After 20 days of Mycobacterium tuberculosis culture, 6 strains in HCl group grew. After 26 days of Mycobacterium tuberculosis culture, except PAS group of 6545 strain, the other 5 strains grew. After 31 days of Mycobacterium tuberculosis culture, the size, shape and distribution of the 6 strains in NS group and HCl group were basically the same, while the distribution of the 6 strains in PAS group was less. The growth curves of 6 strains of Mycobacterium tuberculosis treated with normal saline, PAS and HCl showed that the growth trend of PAS group was lower than that of NS group and HCl group. Compared with the difference of Mycobacterium tuberculosis colony growth under normal saline, PAS and HCl treatment, the colony count of Mycobacterium tuberculosis in NS group was higher than that in PAS group(P<0.05), and there was no statistical significance in the growth of Mycobacterium tuberculosis between NS group and HCl group(P>0.05). Conclusion Plasma activated saline has inhibitory effect on Mycobacterium tuberculosis in vitro experiment, showing great potential in anti-tuberculosis treatment, and may play a synergistic role in combined treatment to improve the therapeutic effect.

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备注/Memo

备注/Memo:

基金项目: 西安市科技计划项目(2023JH-YXYB-0355)
通信作者: 屈少毅, Email: 1183788762@qq.com

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更新日期/Last Update: 2025-04-25